Figure 4
From: Regulation of synaptic plasticity and cognition by SUMO in normal physiology and Alzheimer's disease
SUMOylation is impaired by Aβ and in Tg2576 APP-overexpressing mice.
(a) SUMOylation is decreased in APP mice. Representative SUMO2/3 western blots with hippocampi from Tg2576 APP transgenic and wild-type littermate mice. 1-2 mo. n = 6 each WT and APP, 7–8 mo. n = 3 each, 13–14 mo. n = 4 each; 24–26 mo. n = 3 each. ANOVA p = 0.0005; *p < 0.05 vs. 1–2 mo. group. Data presented as means ± SEM, % of control group. (b) The depolarization-induced increase in SUMOylation at the 5 minute time point is not evident in APP mice compared to WT littermate controls. WT n = 6, APP n = 5; *p = 0.012. (c) Exposure to Aβ42 oligomers impaired the depolarization-induced SUMOylation at the 5 minute time point. Vehicle and Aβ-treated n = 6 each; **p = 0.01. Data presented as means ± SEM, % change in high molecular weight conjugation over non-stimulated control. See also Supplementary Figure S4.
