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Figure 1

From: Integrating computational modeling and functional assays to decipher the structure-function relationship of influenza virus PB1 protein

Figure 1

Structural modeling of influenza A virus PB1 protein.

(A) The modeling flowchart. (B) Selection of the PB1 core region 110–637 as initial seed segment to search its potential structural templates. Four conserved residues (D305, G406, D445 and K481) on the RdRP motifs (motif A to D) are highlighted in red. The binding regions of PA and PB2 on PB1's N and C terminuses are labeled in gray. NLS, Nuclear Localization Signal; POL, Polymerase. (C–D) The comparison of the threading results by using (C) or not using (D) functional site constraints. The percentages of RdRP and Non-RdRP for top 5, 10 and 20 templates were presented in black and gray. (E) The sequence alignment between influenza A PB1 segment 71–612 and the selected template, Norwalk Virus (NV-) RdRP. Five conserved motifs (preA, A, B, C and D) were highlighted. Amino acids are colored according to its polarity: basic amino acids in blue, acidic in red.

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