Figure 2 | Scientific Reports

Figure 2

From: Integrating computational modeling and functional assays to decipher the structure-function relationship of influenza virus PB1 protein

Figure 2

Characteristics of modeled PB1 structure.

(A) The overall view of the model. Three structural domains, “Fingers”, “Palm” and “Thumb” domain were separated with dash lines approximately. Five conserved motifs (preA, A, B, C and D) were shown as flat ribbons colored with purple, blue, drab, green and orange respectively. TBC (template binding and catalytic) channel is indicated in the central of the model. 40 newly identified residues constituted the TBC channel were mapped on the PB1 model, presented side-chains with red. (B) The distribution of non-conserved sites (highlighted by green mesh) on the PB1 model. The structure is rotated around Y axis by 180° refer to (A). The template binding and catalytic (TBC) channel and NTP tunnel are indicated by two curved arrows. The docking of RNA duplex composed of template RNA (blue) and synthesized RNA chains (orange) was based on the RdRP-RNA complex structure of the Norwalk virus (PDB ID: 3BSN). The sites highlighted are the same to that in (A). (C–D) Electrostatic variation from negative to positive charges presented with the color differences from red to blue gradually. Three regions are highly positively charged, designated as positive charge patch (PCP).

Back to article page