Figure 4 | Scientific Reports

Figure 4

From: Integrating computational modeling and functional assays to decipher the structure-function relationship of influenza virus PB1 protein

Figure 4

Effects of mutations in the PB1 subunit on polymerase activity and RNA synthesis.

(A) The location of all selected sites on the PB1 model. Sites colored red if its polymerase activity is less than 1% of that of wild type PB1 as shown in (B), while others blue. Circled sites indicate that the locations of these sites are around the template binding and catalytic channel. (B) The polymerase activity of influenza A in the presence of the indicated PB1 mutants. The activity detected with samples contained wild type PB1 (WT) was set to 100% and a transfection mixture with the omission of PB1 (-PB1) used as negative control. (C) In vivo RNA synthesis mediated by PB1 mutants. In vivo primer extension of vRNA, mRNA and cRNA isolated from 293T cells, as indicated above the line, transfected either with pPolI-CAT-RT and pcDNA3.1 (indicated by “C”), with pPolI-CAT-RT, pcDNA-PB2, pcDNA-PA (-PB1), or with pPolI-CAT-RT, pcDNA-PB2, pcDNA-PA and wild type (WT) or mutant pcDNA-PB1. Signals of vRNA, mRNA, cRNA and 5 s RNA, used as control, are indicated.

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