Figure 4
Fluorescence imaging of large mouse brain sections by APOM with selective plane illumination.
(a) A picture of centimeter-scale fluorescently labeled mouse brain slices embedded in polymer. No special sample preparation is required for APOM. The thickness of the brain slices shown in (a, b, c, e) is about 32 µm and the thickness of the brain slice shown in (d, f) is about 55 µm. (b) A normal plane image of the brain slice in the region, as marked by a yellow dot in (a). The size of the yellow dot is larger than the area shown in (b) for better visibility of the marking dot. The dashed line in (b) indicates the y position of the cross-section shown in (c). (c) An axial plane image of a cross-section of the brain slice as labeled in (b). (d) An axial plane image of a thicker brain slice. (e) and (f) show two 3D fluorescent images of the mouse brain sections obtained by stacking a series of axial plane images together. (e) shows a dense regime while (f) shows a more sparsely stained one. The scale bars are 1 cm in (a), 10 µm in (b-d), respectively.
