Figure 4
Deletion of gut G- bacteria alleviated ConA-induced liver and intestine damage.
Treated mice received oral gentamycin (1 × 104 U/g) for one week followed by injection of ConA (10 mg/kg) and were sacrificed 12 h after ConA administration (n = 8). (a) The ALT, AST levels and representative hepatic H&E images (200×). (b) Systemic endotoxin levels. (c) The percentage of hepatic DCs and NKT, NK and T cells was determined by flow cytometry. (d) ELISA of TNF-α and IL-12 expression in hepatic tissue. (e) Representative intestinal H&E images (200×). (f) Intestinal γδ T, DC, NKT and NK cell ratios were determined using flow cytometric analysis. (g) ELISA assessment of the intestinal TNF-α and IL-12 expression. (h) Lymphocytes were isolated from PPs, The percentages of DCs and T cells were assessed after gentamycin and ConA administration. Data presented are the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, Mann-Whitney U-test.
