Figure 6
Pathogenic bacterial antigens enhanced NKT cell cytotoxicity via DC activation in vitro.
(a) NKT cells from the liver were stimulated for 48 h in vitro. Cytotoxic assay with target TLR2 cells denoted as the PI-positive rate as analyzed by flow cytometry. The TNF-α level in the supernatant was detected by ELISA. (b) NKT cell cytotoxicity was detected after anti-TNF-α antibody neutralization, co-cultured with CD11c+ cells and Salmonella. (c) Schematic representation of immune cell activation in ConA-induced hepatic injury controlled by intestinal microbiota. Data presented are the means ± SD. *P < 0.05 **P < 0.01, one-way ANOVA.
