Figure 2
From: A novel method for synthetic vaccine construction based on protein assembly
Design and generation of SpyTag/SpyCatcher-based DC-targeted synthetic vaccine.
(a) Schematic diagram of the synthetic vaccine design and assembly. The cDNA encoding the single chain of αDEC205 was genetically fused to SpyTag at the C-terminus and the antigen containing the OT1 epitope and TBEV ED3 was fused to the C-terminus of SpyCatcherΔN. Once mixed, an amide bond efficiently forms between SpyTag and SpyCatcher. (b) Synthetic vaccine production and purification. Purified αDEC205-SpyTag was mixed with SpyCatcherΔN-OVA8-TBEV ED3 at a molar ratio of 1:1.5 at 4°C for 2 h. The assembled αDEC205-Sc-OVA8-ED3 adduct was purified by Protein A chromatography. Both the efficiency of the reaction and the purified adduct can be analyzed by SDS-PAGE.
