Figure 2 | Scientific Reports

Figure 2

From: Pleiotropy of the de novo-originated gene MDF1

Figure 2

Inhibition of Snf1p by Mdf1p is responsible for the promotion of fermentation.

(A) A Co-IP assay using an Mdf1p-3HA-tagged overexpression strain confirmed that Mdf1p and Snf1p interact in lag phase. The resultant Co-IP products were tested via western blotting using a Snf1p antibody and 10% yeast samples before precipitation were used as an input control. (B) The expression levels of all of the glucose-repressed genes in the MDF1 strain were reduced in comparison with the wild-type strain in quantitative RT-PCR assays. ACT1 was used as an internal control.

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