Figure 5
From: RILP interacts with HOPS complex via VPS41 subunit to regulate endocytic trafficking
C-terminal region of VPS41 contributes to the interaction between RILP and HOPS complex.
(A). Purified His-VPS41 or His-Vps33 recombinant protein was incubated with immobilized GST-RILP to show RILP directly interacts with VPS41, but not VPS33. (B). Hela cells were co-expressed with GFP-RILP and myc-VPS41, myc-VPS41(1–427) or VPS41(428–855), respectively. Immunofluorescence microscopy revealed that RILP can recruit wild type VPS41 and VPS41(428–855), but not VPS41(1–427). (C). Pulldown experiments showed that RILP specifically binds to VPS41 through its C-terminal region(428–855aa). Un-cropped blots/gels are presented in Supplementary Figure s10. Bar = 20 μm.
