Table 2 Summary of the diffraction data of the crystals grown in the presence and absence of SDB microspheres

From: Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality

 

Diffraction data statistics

Protein

Condition

Resolution (Ã…)

I/σI

Rmerge (%)

Mosaicity (°)

Completeness (%)

Concanavalin A

SDB1

50.00–1.54 (1.57–1.54)

34.52 (2.25)

8.70 (87.40)

1.04

100.00 (99.90)

 

Control

50.00–2.10 (2.14–2.10)

15.44 (1.38)

10.70 (95.60)

2.77

78.90 (87.00)

Proteinase K

SDB1

50.00–1.20 (1.22–1.20)

31.86 (3.27)

10.80 (64.40)

0.41

98.60 (89.50)

 

Control

50.00–1.19 (1.21–1.19)

38.20 (2.44)

98.90 (90.90)

0.34

98.90 (90.90)

Thaumatin

SDB1

50.00–1.20 (1.22–1.20)

65.68 (2.37)

5.90 (71.70)

0.13

98.70 (85.00)

 

Control

50.00–1.49 (1.52–1.49)

51.81 (3.33)

9.20 (90.50)

0.80

99.80 (97.20)

ThiM

SDB1

50.00–1.97 (2.09–1.97)

12.70 (2.60)

6.90 (50.30)

0.15

94.20 (77.90)

 

Control

-

-

-

-

-

TCS

SDB1

50.00–1.01 (1.03–1.01)

(46.56) 6.81

7.00 (29.10)

0.12

98.50 (88.60)

 

Control

-

-

-

-

-

HSP90

SDB1

50.00–1.43 (1.45–1.43)

(46.36) 2.72

5.90 (91.50)

0.22

98.60 (98.20)

 

Control

50.00–2.65 (3.34–2.65)

(33.10) 6.40

26.10 (91.10)

1.15

100.00 (97.30)

Glucose isomerase

SDB1

50.00–1.90 (2.01–1.90)

18.60 ± 4.40 (9.90 ± 3.50)

12.30 ± 7.50 (22.20 ± 11.30)

0.20 ± 0.06

98.92 ± 0.59 (95.52 ± 3.21)

 

Control

50.00–1.90 (2.01–1.90)

12.40 ± 4.30 (5.60 ± 3.50)

13.50 ± 3.40 (58.90 ± 30.20)

0.40 ± 0.14

98.67 ± 0.84 (93.80 ± 5.48)

MLI

SDB1

50.00–2.79 (2.97–2.79)

20.0 ± 1.90 (3.70 ± 1.20)

13.70 ± 3.20 (85.0 ± 23.8)

0.31 ± 0.06

99.37 ± 0.29 (96.63 ± 1.96)

 

Control

50.00–2.79 (2.97–2.79)

30.90 ± 10.60 (7.80 ± 6.10)

8.60 ± 2.90 (49.50 ± 27.80)

0.17 ± 0.05

99.43 ± 0.26 (97.13 ± 1.57)

  1. Note: Statistics for the glucose isomerase and MLI single data sets are summarised in Supplementary Table 1.