Figure 5
From: Capture-stabilize approach for membrane protein SPR assays
Capture-stabilize CD52 VLP on C1 chip for anti-CD52 antibody analysis.
(a). SDS-PAGE analysis of samples produced by HEK293FT cell transient expression. Lanes: 1. Sf9 cell negative control membrane prep, 2. Purified CD52 positive control protein produced in Sf9 cell, 3. HEK293FT negative control total cell lysates, 4. HEK293FT total cell lysates transiently expressing CD52, 5. A negative control VLP prep, 6. CD52 VLP prep from HEK293FT cells. (b). Western blotting with anti-CD52 antibody Alemtuzumab. The samples are the same as shown in panel A. (c). Biacore kinetics assay sensorgrams of anti-CD52 mAbs generated using CD52 VLPs captured and stabilized on a C1 chip surface.
