Table 3 Kinetics assay on whole receptor surface with two different receptor densities
From: Capture-stabilize approach for membrane protein SPR assays
Receptor Density | Sample | Data | ka (1/Ms) | kd (1/s) | KD (M) |
|---|---|---|---|---|---|
300RU | 16D7 | Mean | 1.94E+05 | 4.09E-05 | 2.10E-10 |
CV (%) | 2.07E-02 | 1.79E-01 | 1.72E-01 | ||
79E7 | Mean | 4.86E+05 | 4.05E-04 | 8.33E-10 | |
CV (%) | 3.64E-02 | 2.70E-02 | 1.88E-02 | ||
CXCL13 | Mean | 6.78E+06 | 5.46E-03 | 8.12E-10 | |
CV (%) | 2.38E-01 | 1.92E-01 | 6.12E-02 | ||
MAB190 | Mean | 7.18E+05 | 3.30E-04 | 4.59E-10 | |
CV (%) | 3.80E-02 | 1.40E-01 | 1.22E-01 | ||
700RU | 16D7 | Mean | 1.32E+05 | 4.52E-05 | 3.40E-10 |
CV (%) | 2.97E-02 | 2.64E-01 | 2.33E-01 | ||
79E7 | Mean | 3.64E+05 | 2.06E-04 | 5.65E-10 | |
CV (%) | 3.62E-02 | 1.27E-01 | 9.41E-02 | ||
CXCL13 | Mean | 7.82E+06 | 8.78E-03 | 1.13E-09 | |
CV (%) | 1.43E-01 | 9.66E-02 | 1.06E-01 | ||
MAB190 | Mean | 4.80E+05 | 3.93E-04 | 8.20E-10 | |
CV (%) | 5.78E-02 | 8.00E-02 | 5.56E-02 |
