Figure 1
From: Natural RNA interference directs a heritable response to the environment
Inheritance of a response to environmental conditions.
Single-channel microarray analysis of 4-cell stage embryo mRNAs shows inheritance of temperature responses. Scatterplot of 15,208 genes: x-axis, generation 2 (G2); y-axis, generation 3 (G3). Values are ratios of geometric mean signal (25°C treatment/constant 20°C, six 50-embryo replicates each condition). Numbers in each quadrant count genes whose mRNA levels differ between treatment and control in both generations (>2× and ANOVA/Student's TP < 0.01, independently in each generation, larger dots; note this figure uses the statistics as a filter, not as a test). Pale dots are the lowest-signal 1/6 of genes and any genes with >4× the average inter-replicate variance. At right margin, G3 replicate RT-QPCR of additional experiments.
