Figure 2
From: Natural RNA interference directs a heritable response to the environment
RT-QPCR of multigenerational experiments.
Values are mRNA abundances relative to constant 20° control (dotted line) for multiple generations after one (black) or two (magenta) generations growth at 25°, geometric mean ± SEM. Two-tailed, paired T-test for difference with 20° control (shown for last two generations only), *P < 0.01 **P < 0.0001. To eliminate males and for more accurate staging, we transferred L4 hermaphrodite larvae from plate to plate (by platinum pick) instead of embryos. See Supplementary Table S5 for statistical details.
