Figure 3
From: Natural RNA interference directs a heritable response to the environment
Temperature-responsive early embryonic mRNAs are highly enriched for targets of endogenous 22G RNAs.
Circles labeled “G2” and “G3” represent genes whose mRNA levels in each generation differ >2× between treatment and control and ANOVA/Student's TP < 0.01 (see Figure 1). All P values are from cumulative hypergeometric distributions. Unless noted otherwise, P values represent the probabilities of the observed or greater overlap between the genes highlighted in Figure 1 and the sRNA set, relative to all genes on the array that are also represented among the annotations used by the published studies (15,19735 and 15,12036). The circle representing the set of all genes is omitted despite being required for a complete Venn diagram. The P values shown in parentheses below are for enrichment relative to the “G2” set alone, restricting analysis to only the genes identified as differing in G2. See Supplementary Notes for calculations. (a) Overlap with oocyte antisense sRNAs35 occurring at >50 (left), >100 (center) and >200 (right) reads per million. P = 6 × 10−8 (7 × 10−6), 7 × 10−10 (4 × 10−6) and 7 × 10−9 (4 × 10−5), respectively. (b) Overlap with sRNAs reported to be depleted >2× in all three of a mut-2 strain, a mut-7 strain and a strain with twelve argonaute genes disrupted35. P = 8 × 10−9 (7 × 10−5). (c) Overlap with sRNAs occurring at >10 reads per million in wild-type and depleted >20× in a mut-16 mutant36. P = 1 × 10−10 (1 × 10−5).
