Figure 4
From: Natural RNA interference directs a heritable response to the environment
WAGO class 22G RNAs but not CSR-1 class 22G RNAs are associated with transcripts showing heritable temperature effects.
(a) Among transcripts targeted by 22G RNAs isolated from oocytes35, most are targeted by either WAGO-9 binding7 or CSR-1 binding 22G RNAs42, as previously reported14. (b) CSR-1 binding 22G RNAs tend to target transcripts that are highly expressed in 4-cell stage embryos. Histograms are distributions of fluorescence values on 20°C control microarray hybridizations. Dotted lines are median values for each group of genes indicated. (c) Failure of the temperature-responsive mRNAs highlighted in Figure 1 to overlap with 22G RNAs that co-immunoprecipitate with the germline argonaute CSR-142 (26% of the genes represented on our microarray). P(no overlap) = 1 × 10−3 (P = 2 × 10−4 if analysis is restricted to only transcripts targeted by oocyte 22G RNAs). (d) By contrast, overlap with 22G RNAs that co-immunoprecipitate with the germline argonaute WAGO-97. P(equal or greater overlap) = 2 × 10−5 (P = 0.03 if analysis is restricted to only transcripts targeted by oocyte 22G RNAs).
