Figure 5

Targets of PRG-1 dependent 22G RNAs are more likely than other transcripts to show effects in the following generation.

(a) Among transcripts differing in G2, likely PRG-1 targets are more likely to show a difference in G3, P = 1 × 10⁻⁸. Circle labeled “G2” represents genes whose mRNA levels differ >3× between treatment and control (one-tailed Student's T P < 0.001; this results in an estimated false positive rate for G2 of approximately 1 gene out of 15,208 based on swap tests). Circle labeled “targets of prg-1 dependent 22G RNAs” represents genes for which antisense 22G RNAs are consistently depleted in five comparisons of N2 (wild type) with prg-1 mutants^{23,24,72,73,74} (see Methods). (b) Same analysis as in (a), looking only at targets of oocyte 22G RNAs. Among transcripts differing in G2, likely PRG-1 targets are more likely to show a difference in G3, P = 4 × 10⁻³. (c) Among targets of oocyte 22G RNAs³⁵, predicted 21U RNA targets^23,24 tend to be WAGO targets⁷ and not CSR-1 targets⁴², as previously reported¹⁴.