Figure 3
Decreased BATF mRNA expression in the tumoural region of Squamous cell carcinoma.
(a)- Quantitative Real-time PCR analysis of BATF (Basic leucine zipper transcription factor, ATF-like) mRNA expression in the tumoural and control lung area of patients with ADC (adenocarcinoma) and SCC (squamous cell carcinoma) (N (ADCcontrol) = 21; N (ADCtumoural) = 20; N (SCCcontrol) = 17; N (SCCtumoural) = 17). Data are shown as mean values ± s.e.m. using student's t-test *P,0.05; **P,0.01. (b),(c)- Analysis of BATF protein expression by Immunohistochemistry (IHC) in the tumoural region of ADC (b) as compared to the tumoural region of SCC (c). d- Luciferase measurement of 2 representative mice injected with LL/2-luc-M38 cells. e,f- Quantitative Real-time PCR analysis of Batf and Stat3 mRNA expression in CD4+ T-cells, isolated from the lungs of naïve as compared to tumour bearing wild-type mice and stimulated with anti-CD3 and anti-CD28 antibody. Data are shown as mean values ± s.e.m. using student's t-test *P,0.05 (N = per group).
