Figure 4
Increased pSTAT3 activation in the tumoural region of the lung with adenocarcinoma.
(a)- Quantitative Real-time PCR analysis of STAT3 mRNA expression in the tumoural and control lung area of patients with ADC and SCC (N (ADCcontrol) = 24; N (ADCtumoural) = 24; N (SCCcontrol) = 19; N (SCCtumoural) = 19). Data are shown as mean values ± s.e.m. using student's t-test *P,0.05; **P,0.01. (b)-(d)- Western blot analysis and quantification of protein levels of pY-Stat3 relative to Stat3 (b), pS-Stat3 relative to Stat3 (c) and Stat3 relative to Actin (d) in the control and tumoural lung area of patients with ADC as well as SCC (pY-Stat3: N (ADC) = 7, N (SCC) = 7; pS-Stat3:N (ADC) = 7, N (SCC) = 7; Stat3: N (ADC) = 7, N (SCC) = 7). Data are shown as mean values +/- s.e.m. using student's t-test *P,0.05. e-h-Representative western blot for pY-Stat3, pS-Stat3, Stat3 and Actin in the control and tumoural lung area of patients with ADC as well as SCC.
