Figure 1

OAG-induced TRPC6 versus hyperforin-induced currents.

Inward and outward currents at −80 and +80 mV, respectively, from HEK-293 cells, stably expressing TRPC6 cDNA (HEK-TRPC6; a, c), non-transfected HEK (HEK) cells (e) and primary mouse cortical microglial cells (g). As indicated by the bars 100 μM OAG or 100 μM flufenamic acid (FFA; a), 1, 3 or 10 μM hyperforin supplied as dicyclohexylammonium (DCHA) salt (c, e) and 10 μM hyperforin supplied as free acid in methanol (MtOH; g) were applied. Currents were normalized to the cell size and basic currents before compound application were subtracted. The corresponding current-voltage relationships (IVs) are displayed in (b, d, f) and (h). Data represent means ± S.E.M. of the indicated number of experiments (cells).