Figure 1

IL-17 and IL-17R are expressed in the DG of hippocampus.

(A). Real-time RT-PCR results show expression of IL-17 messenger relative to GAPDH in WT and IL-17 KO DG tissues. (B). Expression of IL-17R at protein level in WT and IL-17 KO DG tissues was confirmed using ELISA. (C–D). Results from FACS intracellular staining of cultured astrocytes, microglia, neurons and NPCs show astrocytes as a major source of IL-17. (E). IL-17R mRNA is expressed in cultured WT and IL-17 KO NPCs. (F–G). FACS analysis shows expression of IL-17R in cultured WT and IL-17 KO NPCs. Data were obtained from three individual experiments performed in triplicate. Means ± SEM; **p < 0.01.