Figure 2

Genetic deficiency of IL-17 increases neurogenesis in the adult DG of hippocampus.

(A). Confocal images of the DG showing BrdU (red) and DAPI (blue) staining at 2 or 4 weeks after injection of BrdU to WT (top) and IL-17 KO (bottom) mice. (B). Quantification of BrdU-immunopositive cells at 2 weeks (left; 2 WPI) and 4 weeks (right; 4 WPI) post-BrdU injection (mean ± SEM; n = 4 mice per condition). Scale bar, 100 μm. (C). Confocal images of BrdU⁺DCX⁺ cells showing BrdU and DCX immunoreactivity as merged images at 2 weeks after 4 days of daily BrdU injections. (D). Number of new immature neurons (BrdU⁺/DCX⁺) in the dentate subgranular zone/granule cell layer at 2 weeks after 4 days of daily BrdU injections. (E). Confocal images of BrdU⁺NeuN⁺ cells showing BrdU and NeuN immunoreactivity as merged images at 4 weeks after 4 days of daily BrdU injections. (F). Number of new immature neurons (BrdU⁺/NeuN⁺) in the dentate subgranular zone/granule cell layer at 4 weeks after 4 days of daily BrdU injections. Means ± SEM; n = 6 for WT and IL-17 KO, respectively. **p < 0.01.