Figure 5
Intracellular ROS estimation and apoptotic related gene expression analyses (A) The level of intracellular ROS after D2O + N2 plasma exposure for 0 min (control), 3 min and ROS scavenger trolox + 3 min treatment. All values are expressed as mean fluorescence intensity (MFI) and ± SD in triplicates; (B) Qualitative analysis of the intracellular ROS level after plasma treatment through fluorescence microscopy using fluorescent probes H2DCFDA; (C) The relative value of mRNA expression of nine apoptosis and related genes of G361melanocytes cancer cells after plasma exposure for 3 min with and without presence of trolox. The relative mRNA expression of p53, Caspase-8, ATM, Bax and NOX(1-5) subfamily were measured by real-time RT-PCR on day 1 and 18s rRNA was used as a reference gene. All values are expressed as (MFI) and ± SD in triplicates. Students' t-test was performed to control (* denotes P<0.05 and ** denotes P<0.01).
