Figure 7

Protein oxidation and extracellular protein modification analyses (A) Mb; (B) Hb proteins were exposed to D₂O + N₂ plasma for different time intervals through circular dichroism; (C) SDS gel electrophoresis of treated Mb and Hb proteins for 5 min and 30% (v/v) H₂O₂ treatment; (D) Production of carbonyl content measured the treated protein with D₂O + Nitrogen plasma for without treatment (control), 3 min, 5 min and 50 μM H₂O₂.Each value is the average of three technical replicates. Students' t-test was performed to control (* denotes P<0.05 and ** denotes P<0.01).