Figure 2

Itgb3 signaling mediates the effects of bone substrate on osteoclastogenesis.
(a) Detailed microarray analysis of itgb3 expression in cells cultured for 1, 3 and 5 days on plastic or calvaria (bone), in the presence (+RL) or absence (−RL) of RANKL. (b) Phalloidin stain showing distinctive actin morphology of itgb3 deficient BMM-derived osteoclasts differentiated on bone. (c) Hierarchical clustering heat map of mouse BMM-derived bone induced osteoclast expression profiles regulated by itgb3.