Figure 4 | Scientific Reports

Figure 4

From: Comprehensive profiling analysis of actively resorbing osteoclasts identifies critical signaling pathways regulated by bone substrate

Figure 4

Regulation of the S-1-P pathway by RANKL, bone and itgb3.

(a) GSEA analysis showing that RANKL, bone and itgb3 each independently modulates this pathway. (b) Detailed microarray analysis of SphK1 and Spns2 probe sets in cells cultured for 1, 3 and 5 days on plastic or calvaria (bone), in the presence (+RL) or absence (−RL) of RANKL. (c) Pathway analysis showing S-1-P pathway components that are co-coordinately regulated by RANKL, itgb3 and bone substrate during osteoclastogenesis. (d) Immunohistochemistry analysis showing SphK1 expression in osteoclasts on the bone surface in sites of rheumatoid arthritis bone erosions.

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