Figure 5 | Scientific Reports

Figure 5

From: Comprehensive profiling analysis of actively resorbing osteoclasts identifies critical signaling pathways regulated by bone substrate

Figure 5

Inhibition of SphK1 represses RANKL-induced murine and human osteoclastogenesis on plastic and bone.

(a) Inhibition of RANKL-induced murine osteoclast formation by Sphk1 inhibitors (N′,N′-DMS and Compound 54) as assessed by quantitation of TRAP-positive multinucleated cells (n = 3, * = p < 0.05 compared to DMSO control). (b) Pit formation assay showing that the inhibitors (at 2.5 uM) inhibit osteoclast mediated bone resorption. (c) Effects of SphK1 inhibitors on RANKL-induced expression of Cathepsin K as assessed by quantitative PCR (n = 3, * = p < 0.05 compared to DMSO control). (d) Inhibition of RANKL-induced human monocyte-derived osteoclast formation by Sphk1 inhibitors (N′,N′-DMS and Compound 54) as assessed by quantitation of TRAP-positive multinucleated cells (representative experiment of three).

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