Table 1 Comparison of microarray and quantitative RT-PCR expression analysis for selected genes. Wild-type (WT) and integrin beta 3 deficient (Itgb3KO) cells were cultured on plastic or bone substrate with M-CSF and RANKL for 5 days. For each analysis, expression in wild-type cells (WT) cultured on plastic is set to a value of 1.00 and relative values and standard deviations calculated. Primer sequences are given in the Methods Section

From: Comprehensive profiling analysis of actively resorbing osteoclasts identifies critical signaling pathways regulated by bone substrate

 

   Microarray Analysis

   Quantitative RT-PCR

Gene

WT Plastic

WT Bone

Itgb3KO Plastic

Itgb3KO Bone

WT Plastic

WT Bone

Itgb3KO Plastic

Itgb3KO Bone

AnxA8 (1425789_s_at)

1.00 (0.60)

12.86 (0.80)

0.46 (0.28)

13.33 (1.98)

1.00 (.034)

66.47 (16.98)

1.34 (0.04)

73.96 (19.40)

AnxA8 (1417732_at)

1.00 (0.33)

14.40 (0.80)

0.64 (0.23)

15.88 (1.87)

    

UCHL1 (1448260_at)

1.00 (0.04)

1.69 (0.06)

0.02 (0.005)

0.04 (0.01)

1.00 (0.13)

2.09 (0.34)

0.02 (0.01)

0.06 (0.03)

FKBP9 (1423677_at)

1.00 (0.08)

1.58 (0.12)

0.19 (0.04)

0.16 (0.04)

1.00 (0.41)

2.91 (1.23)

0.20 (0.09)

0.21 (0.19)

FKBP9 (1437687_x_at)

1.00 (0.02)

1.49 (0.03)

0.15 (0.002)

0.13 (0.01)

    

SphK1 (1451596_a_at)

1.00 (0.03)

1.52 (0.16)

0.60 (0.06)

0.80 (0.08)

1.00 (0.66)

1.54 (0.48)

0.57 (0.15)

0.83 (0.51)

Spns2 (1451601_a_at)

1.00 (0.09)

1.69 (0.07)

1.62 (0.08)

1.48 (0.21)

1.00 (0.26)

2.39 (0.41)

1.51 (0.70)

1.76 (0.39)

Back to article page