Figure 1
Polysaccharides preserved photoreceptor morphology in the rd10 retina at P26.
(a–c). Representative photomicrographs of hematoxylin and eosin (h&e) stained ultra thin retinal sections. The thinner ONL is observed in PBS-treated rd10 retina (c), while polysaccharides preserved the thickness of the ONL (b). Retinas from WT mice at the same age are used as a comparison (a). (d). Quantification of the ONL thickness, measured in numbers of photoreceptor nuclei per column. Results are presented as means ± SDs; n: number of retinal sections in each group. ** p < 0.01. (e–g). The OS/ISs of cones are revealed by an antibody against red/green opsin (red). Cone's OS/ISs in polysaccharides- treated rd10 mice ((f), red) appear longer than those in PBS-treated rd10 mice ((g), red). Insets illustrate highly magnified image of the OS/ISs of cones from the boxed regions. (h). Plot of the length of OS/ISs. Results are presented as means ± SDs; n: number of retinal sections in each group. ** p < 0.01. (i–k). Retinal whole-mounts. Confocal images of cone from the superior region of the retina of WT (i), polysaccharides- (j) and PBS- treated rd10 mice (k) are shown. (l). Quantification of cone density. Results are presented as means ± SDs; n: number of flat mounted retinas in each group. ns, not significant, * p < 0.05. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer; OS: outer segment; IS: inner segment.
