Figure 3
From: Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C
Angiogenin expression in viral hepatitis and hepatocellular carcinoma.
(a) Normalized ANG mRNA levels from previously generated liver microarray data14 from uninfected subjects (n = 6), non-malignant (n = 7) and liver cancer (n = 8) tissue from HBV-infected subjects and non-malignant (n = 11) and cancer tissue (n = 15) from HCV-infected subjects. **P < 0.01; ***P < 0.005, calculated by Mann-Whitney U-test. (b) Scatter plot of the levels of 5′ tRHs (RT-qPCR, -dCT normalized to RNU48) and ANG mRNA (microarray). 5′ tRHGly (“Gly[C/G]CC”): uninfected subjects (n = 7), non-cancer (n = 7) and cancer (n = 8) liver tissue from chronic hepatitis B subjects and non-cancer (n = 11) and cancer (n = 15) liver tissue from chronic hepatitis C subjects; 5′ tRHVal: uninfected subjects (n = 6), non-cancer (n = 7) and cancer (n = 8) liver tissue from chronic hepatitis B subjects and non-cancer (n = 11) and cancer (n = 15) liver tissue of chronic hepatitis C subjects. (c) Scatter plot of the levels of 5′ tRHs (RT-qPCR, -dCT normalized to RNU48) and ANG protein expression (normalized to β-actin) determined by immunoblot analysis. (d) Immunohistochemistry staining for ANG in formalin-fixed non-tumor (NT) and tumor tissue (T) from HBV-infected subject #10. (Right) Magnified view of non-tumor (NT). (e) ANG staining in adjacent tumor nodules (T1 and T2) and in non-tumor (NT) tissue from HCV-infected subject #7.
