Figure 1 | Scientific Reports

Figure 1

From: Meiotic interstrand DNA damage escapes paternal repair and causes chromosomal aberrations in the zygote by maternal misrepair

Figure 1

Photomicrographs of mouse spermatocytes, sperm and zygotes.

(A–C) Mouse MI and MII spermatocytes after DAPI staining. (A) Normal MI spermatocyte. (B) MII spermatocyte with on chromatid fragment (arrowhead). (C–E). Mouse sperm after hybridization with probe specific for the centromeric region of chromosome 2 labeled with FITC, the telomeric region of chromosome 2 labeled with rhodamine and the subcentromeric region of chromosome 8 labeled with rhodamine and FITC. (C) Normal mouse sperm showing three spots each of a different color. (D). Sperm with the duplication of the centromeric region of chromosome 2 (two red spots). (E) Diploid sperm (two spots for each of the three probes). (F–h) Mouse zygotes after hybridization with chromosome-specific painting probes for chromosomes 1, 3, 5, X and Y labeled with FITC and chromosomes 2, 4, 6, X and Y labeled with biotin and signaled with Texas red. (F) Normal 1-Cl zygote metaphase with X-bearing sperm-derived chromosomes on the left. (G) Paternal chromosomes from a 1-Cl zygote showing a reciprocal translocation (arrows) involving one chromosome painted in green and one unpainted chromosome. (H) Paternal chromosome from a 1-Cl zygote with extensive chromosomal damage involving painted and unpainted chromosomes.

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