Table 1 Chromosome structural aberrations (CSA) in first-cleavage (1-Cl) zygotes after exposure of male mice to 7.5 mg/kg MLP

From: Meiotic interstrand DNA damage escapes paternal repair and causes chromosomal aberrations in the zygote by maternal misrepair

      

DAPI Analysisc

PAINT Analysisc

Treatment daya

No of. miceb

Stage at treatment

No. of zygotes analyzed

No. of zygotes with CSA

% ± S.E.

Chromosome-type

Chromatid-type

Cell equivalents

Total

Controls

45 (91)

 

282

3

1.1 ± 0.6

0.014 ± 0.008

0

166

0.018 ± 0.016

3

30 (60*)

Epidydimal sperm

139

15

10.8 ± 0.6*

0.237 ± 0.153

0.007 ± 0.008

82

0.219 ± 0.096

7

53 (57*)

Testicular sperm

230

41

17.8 ± 2.0#

0.426 ± 0.124

0.004 ± 0.005

135

0.488 ± 0.179

23

52 (81)

Diplotene spermatocytes

293

188

64.2 ± 3.8#

1.683 ± 0.158

0.007 ± 0.007

170

1.929 ± 0.207

37

27 (89)

Dividing spermatogonia

216

12

5.6 ± 1.1§

0.093 ± 0.008

0

127

0.064 ± 0.013

49

37 (92)

Stem cells

167

2

1.2 ± 0.6

0.006 ± 0.007

0.006 ± 0.005

93

0

  1. aPrior to mating.
  2. bPercent of matings in parenthesis.
  3. cNumber of CSA per metaphase (mean ± Standard Error).
  4. *p < 0.001 versus controls (Chi-square).
  5. #p < 0.0001 versus controls (Chi-square).
  6. §p < 0.05 versus controls (Chi-square).
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