Table 2 Frequencies of sperm with structural and numerical abnormalities in B6C3F1 mice after melphalan treatment of meiotic cellsa
Genotype | Fluorescent pattern | Controlsb | Melphalanb | P value |
|---|---|---|---|---|
No. Mice | 3 | 4 | ||
No. cells scored | 30,288 | 40,208 | ||
Sperm with structural aberrations | ||||
Centromeric duplication | CCT8 | 0.0 | 1.2 ± 0.5 | 0.05 |
Centromeric deletion | OT8c | 0.0 | 1.0 ± 1.0 | 0.08 |
Telomeric duplication | CTT8 | 2.6 ± 0.7 | 1.5 ± 1.2 | 0.28 |
Telomeric deletion | CO8 | 3.0 ± 1.0 | 1.0 ± 0.7 | 0.06 |
Total Structural | 5.6 ± 1.5 | 4.7 ± 1.5 | 0.61 | |
Sperm with numerical abnormalities | ||||
Disomy 2 | CCTT8 | 1.0 ± 0.0 | 0.7 ± 0.5 | 0.73 |
Nullisomy 2 | OO8 | 1.0 ± 0.0 | 3.0 ± 0.8 | 0.07 |
Disomy 8 | CT88 | 1.7 ± 0.7 | 3.7 ± 0.5 | 0.11 |
Nullisomy 8 | CTO | 2.0 ± 1.0 | 2.5 ± 1.7 | 0.66 |
Total | 5.6 ± 1.5 | 9.9 ± 1.5 | 0.04 | |
Diploid sperm | CCTT88 | 6.6 ± 4.2 | 10.7 ± 2.2 | 0.07 |
Sperm with complex abnormalities | 1.0 ± 0.6 | 0.7 ± 0.5 | 0.73 |
