Figure 3
From: Distinct p21 requirements for regulating normal and self-reactive T cells through IFN-γ production
p21 overexpression reduces IFN-γ production in B6/lpr-p21tg effector and memory CD4+ T cells.
Lymph node CD4+ T cells from 4-month-old B6/lpr, B6/lpr-p21tg, B6 and B6-p21tg mice were analyzed by flow cytometry. (a) Intracellular cytokine analysis showed reduced frequency of IFN-γ-producing cells in CD44hiCD62Lhi CD4+ T cell populations from B6/lpr-p21tg compared with B6/lpr mice. Data are from one representative experiment (n = 4 mice; p < 10−2) of 2 performed. Percentages of IFN-γ-producing cells were similar for B6-p21tg and B6 CD44hiCD62Lhi CD4+ T cells. (b) The frequency of IFN-γ-producing CD44hiCD62Llo CD4+ T cells was lower in B6/lpr-p21tg compared with B6/lpr mice. Data are from one representative experiment (n = 4 mice; p < 10−2) of 2 performed. Percentages of IFN-γ-producing CD44hiCD62Llo CD4+ T cells were similar for B6-p21tg and B6 mice. (c) Small CD44hi cells showed reduced frequency of IFN-γ-producing cells in B6/lpr-p21tg compared with B6/lpr mice. Percentages of IFN-γ-producing small CD44hi CD4+ T cells were similar for B6-p21tg and B6 mice.
