Figure 8
From: Distinct p21 requirements for regulating normal and self-reactive T cells through IFN-γ production
p21 overexpresson reduces autoimmune disease manifestations in B6/lpr mice.
(a) Anti-DNA IgG levels detected by ELISA in mouse serum at 4 months of age. Anti-DNA IgG production was reduced in B6/lpr-p21tg compared to B6/lpr mice. Values show mean ± SD (n = 9 mice; p < 10−4). (b) Reduced proportions of DN T cells in B6/lpr-p21tg lymph nodes compared to B6/lpr at 5 months of age (n = 4 mice). (c) Reduced cervical lymph node size and weight in B6/lpr-p21tg females compared to B6/lpr at 8 months of age. Values show mean ± SD (n = 10 mice). (d) Western blot showed high transgenic p21 protein levels in DN T cells from 2-month-old B6/lpr-p21tg mice. Endogenous p21 is not expressed in B6/lpr or B6/lpr-p21tg DN T cells. β-actin was used as a loading control. (e) Intracellular staining showed reduced production of IFN-γ (left) and IL-17 (right) in B6/lpr-p21tg DN T cells. Values show mean ± SD (n = 4 mice; p < 10−2 for both cytokines).
