Figure 5
Iso-GNA causes autophagy-dependent cell death in H460 cells.
(A) Different concentrations of iso-GNA with 0, 2 or 4 mM 3-MA were added to H460 cells for 24 h, then cells were stained with trypan blue to test death ratio. (B) Detection of Beclin1 or Atg7 expression by western blot in Beclin1 or Atg7 knock down H460 cells. (C) Different concentrations of iso-GNA were added to normal, Beclin1 knock down or Atg7 knock down H460 cells for 24 h, then cells were stained with trypan blue to test death ratio. (D) and (E) Different concentrations of iso-GNA with/without bafilomycin A1 (D) or chloroquine (E) were treated with H460 cells for 24 h, trypan blue dye was employed to stain the cells and analyze the death ratio. Data of three independent tests were shown as means ± s.d. *p < 0.5; **p < 0.01, in comparison with the untreated group. The whole images of western blots are given in Supplementary Figure S9.
