Figure 2
From: Dendritic spine dynamics leading to spine elimination after repeated inductions of LTD
Dendritic spine dynamics (spine generation and retraction) after 3 inductions of cLTD.
(a) Spines generated and retracted during indicated periods, after 3 inductions of cLTD. Two consecutive images were compared and both generated and retracted spines are identified and expressed as the percentages of the total number of spines that existed in the prior image. The dendritic segments examined are 20 from 9 independent 3× mock cultures and 23 from 10 independent 3× cLTD cultures. Significance values are P = 8.7 × 10−5, t(40) = 4.4 for ***1 and P = 0.047, t(41) = 2.0 for *2 in paired t-test. (b and c) Fate of individual spines that existed at PS day–1 (see Fig. 1b for timeline of experiment). Representative series of images is shown in (b). The plotting shown in (c) indicates the number of spines which were present at PS day-1 and remaining at PS day 6, 13 and 20. The spines newly generated after PS day-1 were not included. Note that disappearance of spines occurred immediately after the 3rd cLTD (PS day 6 means 4 days after the 3rd cLTD). After PS day 6, disappearance of spines occurred in a similar rate in both 3× mock and 3× cLTD groups. The dendritic segments examined are 19 from 6 independent 3× mock cultures and 19 from 8 independent 3× cLTD cultures. P value is 4.9× 10−12 for ***3 in 2-factor factorial ANOVA (F(1,36) = 14.0, P = 6.3 × 10−4) followed by Bonferroni's test.
