Figure 3
From: Dendritic spine dynamics leading to spine elimination after repeated inductions of LTD
Absence of the “raised fluctuation phase” during spine number reduction as confirmed by observation of 1-day interval.
(a) Timeline of examination. The 1st imaging was made 1–4 hr (typically 2 hr) before cLTD induction and the 2nd imaging was made 24 hr after the 1st imaging. (b) Representative series of images. (c) Spine number reduction occurred within 1 day after the 3rd cLTD induction. (d) Dynamics of individual spines. Note that the disappearance of spines occurred only after the 3rd cLTD induction. In (c) and (d), the dendritic segments examined are 26 and 27 from 13 and 13 independent cultures for the 1st cLTD and the 3rd cLTD groups, respectively. Significance values are P = 2.3 × 10−3, t(50) = 3.2 for **1 and P = 1.1 × 10−3, t(52) = 3.5 for **2 in paired t-test.
