Table 2 The experimental design used in this study
Experiment | purpose | experimental strain and enzyme | calcium resources | CO2 concentration (%) |
|---|---|---|---|---|
1 | effects of B. mucilaginosus on wollastonite dissolution | B. mucilaginosus | wollastonite/wollastonite and CaCl2 | 0.039 |
2 | effects of sufficiency or deficiency in calcium on CA gene expression | B. mucilaginosus | wollastonite/wollastonite and CaCl2 | 0.039 |
3 | effects of CO2 concentration on CA gene expression | B. mucilaginosus | wollastonite | 0.039/3.9 |
4 | construction of the heterologous expression vector, induction expression and purification of recombinant protein | E. coli and enzyme | _____ | 0.039 |
5 | effects of PCA4 on wollastonite dissolution | enzyme | wollastonite | 0.039/3.9 |
6 | effects of PCA4 and CO2 concentrations on CaCO3 precipitation | enzyme | CaCl2 | 0.4/3.9/10 |
