Figure 2

Gr33a modulates males' preference for younger mates.

(A) Courtship indices of wild type males, Gr33a^Gal4/+ males, Gr33a^Gal4/Gr33a¹ males, Gr33a¹ males and Gr33a^rescue males (Gr33a^Gal4/Gr33a¹; UAS-Gr33a⁺/+) in courtship choice assays towards younger mates (white) and older ones (grey), respectively. Box-and-whisker plots for CIs show 1–99 percentiles and mean (+), n = 22 for wild type males, 23 for Gr33a^Gal4/+ males, 22 for Gr33a^Gal4/Gr33a¹ males, 22 for Gr33a¹ males and 23 for Gr33a^rescue males. n.s., p > 0.05, three asterisks, p < 0.001, Related-samples Wilcoxon Signed Rank test. (B) Preference indices of wild type males, Gr33a^Gal4/+ males, Gr33a^Gal4/Gr33a¹ males, Gr33a¹ males and Gr33a^rescue males in courtship choice assays. Mean ± standard error of the mean (SEM). n.s., p > 0.05, three asterisks, p < 0.001, Kruskal-Wallis test, Dunn's post-hoc. (C) Total courtship indices of wild type males, Gr33a^Gal4/+ males, Gr33a^Gal4/Gr33a¹ males, Gr33a¹ males and Gr33a^rescue males in courtship choice assays. Mean ± standard error of the mean (SEM). n.s., p > 0.05, Kruskal-Wallis test, Dunn's post-hoc.