Figure 3

Activity of Gr33a neurons is essential for males' preference for younger mates.
(A) Courtship indices of control males (Gr33aGal4/UAS-TNTin) and TNT expressing males (Gr33aGal4/UAS-TNT) in courtship choice assays towards younger mates (white) and older ones (grey), respectively. Box-and-whisker plots for CIs show 1-99 percentiles and mean (+), n = 23 for control males and 22 for TNT expressing males. n.s., p > 0.05, three asterisks, p < 0.001, Related-samples Wilcoxon Signed Rank test. (B) Preference indices of control males and TNT expressing males in courtship choice assays. Mean ± standard error of the mean (SEM). Three asterisks, p < 0.001, Mann-Whitney U test. (C) Total courtship indices of control males and TNT expressing males in courtship choice assays. Mean ± standard error of the mean (SEM). n.s., p > 0.5, Mann-Whitney U test.