Figure 6
From: An ignored variable: solution preparation temperature in protein crystallization

A schematic of crystallization solution preparation.
Before the experiment, the HEWL and NaCl solutions were pipetted into an Eppendorf tube at different positions without mixing (left). Next, the Eppendorf tube with the solutions was maintained horizontally in the temperature controller. After a one-hour treatment, the crystallization solution can be generated by turning the Eppendorf tube upright such that NaCl solution moves downwards and mixes with the HEWL solution (right).