Figure 1

CG2107 mutants and rescue with mammalian CPT2.

(A) Protein sequence alignment of Drosophila melanogaster (DM), Drosophila simulans (Dsim), Drosophila pseudoobscura (Dpse), Danio rerio (danio), gallus gallus (chick), homo sapiens (human), mus musculus (mouse), rattus norvegicus (rat) and consensus CPT2. (B) Region 63A2 of Drosophila melanogaster chromosome 3L, bearing dCPT2 (CG2107), P-element insertions, deficiencies Df(3l)428 and Df(3L)671 spanning the CG2107 locus and the sequences deleted in two newly generated mutants dCPT2 ^δ18 and ^δ3. (C) Eclosion rates of dCPT2 mutant flies with the indicated genotypes in percent of expected (n > = 3 experiments, **** p < 0.0001). Rescue was performed with wild type (rCPT2) or mutant (rCPT2 ^H372A) rat CPT driven by the ubiquitous driver da-Gal4. (D) Western blot from mouse liver or whole fly extract from wild type or rCPT2 expressing flies, stained with anti-CPT2 to confirm ectopic expression in rescue flies (65 kDa band marked with an arrow), or anti-Histone 3 as loading control.