Figure 2
From: Identification of molecular signature of head and neck cancer stem-like cells
HNSCC express CSC markers.
(a) qRT-PCR analyses of CD133 in parental and oralspheres from OSC19 and Cal27. Expression of CD133 was normalized to GAPDH. The results are presented as means of three different experiments with standard errors (**, p < 0.001). (b) qRT-PCR analyses of Nanog in parental and oralspheres from OSC19 and Cal27. Expression of Nanog was normalized to GAPDH. The results are presented as means of three different experiments with standard errors (*, p < 0.05). (c) Cell lysates from parental and oralspheres derived from OSC19 and Cal27 were subjected to Western blot analysis for Nanog using specific antibody. The blot was reprobed with an antibody to GAPDH for comparison of equal protein load. Note that cropped gel images are used in this figure and the gels were run under the same experimental conditions.
