Figure 1 | Scientific Reports

Figure 1

From: Cigarette smoking hinders human periodontal ligament-derived stem cell proliferation, migration and differentiation potentials

Figure 1

Cell proliferation and gene expression analyses of human PDLSC from smokers and non-smokers.

(A) The proliferation of human PDLSC from smokers and non-smokers was measured by MTT assay for 7 days. The data represented was the mean of 3 samples ± standard deviation. Solid line: the smoker group; Dotted line: the non-smoker group. ‘*’: p < 0.05, ‘**’: p < 0.01. (B) The gene expression analysis was performed using the Sybr green PCR master mix on a real-time PCR machine. Genes related to stem cell markers (KLF4 and NOTCH1) and proliferation markers (MYC and PCNA) were examined. GAPDH was used for normalization. The data represented was the mean ± standard deviation.

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