Figure 4

EPR Mobility analysis of integrin β_1a-TMC in LDAO micelles and in POPC/POPG liposomes.

(a) Plot of the reciprocal of the central resonance line width ΔH⁻¹ versus the residue number in micelles (black squares) and in liposomes (red dots). (b) Plot of the inverse spectral second moment of the EPR spectrum <H²>⁻¹ versus the residue number in LDAO micelles (black squares) and in POPC/POPG liposomes (red dots). Sine waves were drawn to illustrate the observed periodicity of ΔH⁻¹ and <H²>⁻¹ indicative of a helical structure in (a) and (b). (c) Correlation diagram of <H²>⁻¹ ~ ΔH⁻¹ of integrin β_1a-TMC in LDAO micelles. Two rectangular boxes were drawn to indicate the two different mobility groups. (d) Correlation diagram of <H²>⁻¹ ~ ΔH⁻¹ of integrin β_1a-TMC in LDAO micelles (black dots) and in POPC/POPG liposomes (red triangles) were plotted in the same scale to show the difference between the two mediums. A blue box was drawn to include almost all residues while two red boxes were drawn as in (c).