Figure 6 | Scientific Reports

Figure 6

From: Selection of Nanobodies that Block the Enzymatic and Cytotoxic Activities of the Binary Clostridium Difficile Toxin CDT

Figure 6

CDTa-specific nanobody l+8 and CDTb-specific nanobody l-15.1g inhibit CDTa+b induced cytotoxicity.

Before addition to cultures of adherent HT29 cells, CDTa and CDTb were preincubated with a 16 fold excess of CDTa- or CDTb-specific nanobodies. Four hours after addition of toxins, cells were fixed and cellular morphology was assessed by differential interference microscopy. The cellular cytoskeleton was stained with the F-actin-staining dye phalloidin-rhodamine, cell nuclei were stained with the DNA staining dye Hoechst 33342. Apotome-images were captured with a Zeiss Axiovert 200M microscope. Control cells (top) were incubated for four hours in the absence or presence of CDTa+b.

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