Figure 3
From: Tuning LeSPL-CNR expression by SlymiR157 affects tomato fruit ripening
Mr VIGS affects expression of LeSPL-CNR and key ripening TF genes.
(a-f) Viral delivery of pre-SlymiR157 and mature SlymiR157, as well as expression of the SlymiR157 target gene LeSPL-CNR and TF genes (LeMADS-RIN, LeHB1, SlAP2a and SlTAGL1) in delayed ripening (DR) and red-ripe (RR) sectors of three different AC fruits injected with PVX/pre-SlymiR157 (a, b, c) or PVX/SlymiR157 (d, e, f) were respectively analysed by qRT-PCR. Relative levels of RNA transcripts were obtained by normalising against the baseline expression levels of the internal control 18S rRNA. qRT-PCR of each biological sample was performed in 3-15 technical replicates. Student's t-tests were carried to evaluate the statistical significance at the level of p ≤ 0.001 (***), 0.01 (**) or 0.05 (*) for each of the specific RNA transcripts between DR and RR sectors of each different biological sample. Although there is variance, the results of these qRT-PCR assays show a similar correlation between the viral delivery of pre-SlymiR157 or mature SlymiR157 and the mRNA levels of ripening TF genes in the DR and RR sectors of fruits tested.
