Figure 5
The interaction of S. Typhi flagella derivatives with macrophages.
The moncytic THP-1 cell line was differentiated into macrophages and infected with S. Typhi expressing one of the three-flagellin variants and the non-flagellated mutant (ΔfliC) (a) Histogram showing the median proportion of recovered (intracellular) bacteria normalized by the inoculum and averaged over six experiments at 1 and 2 hours post infection. Asterisk highlights statistically significant variations by pairwise comparison (p<0.001), error bars represent one standard deviation. (b) THP-1 cells were infected and RNA was isolated for microarray analysis of host mRNA population. Figure shows hierarchical clustering of differentially expressed genes in THP-1 cells infected with the three flagellin derivatives and the non-flagellated mutant (ΔfliC), compared with uninfected THP-1 cells. The cut-off for differentially expressed genes was an absolute fold change >2 and FDR corrected p-value of <0.05. Red/green color scale indicates level of gene expression, red indicates increased gene expression and green indicates reduced gene expression.
